254D
ALTERNATING AND NON-ALTERNATING DG-DC HEXANUCLEOTIDES CRYSTALLIZE AS CANONICAL A-DNA
Experimental procedure
| Source type | ROTATING ANODE |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1992-01-01 |
| Detector | RIGAKU |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 39.670, 45.980, 39.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.900 |
| R-factor | 0.176 * |
| Rwork | 0.197 |
| R-free | 0.18600 |
| RMSD bond length | 0.013 * |
| RMSD bond angle | 3.500 * |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 32.000 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 2937 |
| Completeness [%] | 68.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | pH 7.00, VAPOR DIFFUSION, SITTING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | SPERMINE | ||
| 5 | 1 | 1 | MGCL2 | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 1 (mM) | |
| 2 | 1 | drop | sodium cacodylate | 15 (mM) | |
| 3 | 1 | drop | spermine | 0.5-5 (mM) | |
| 4 | 1 | drop | 1-20 (mM) | ||
| 5 | 1 | drop | MPD | 5 (%(v/v)) | |
| 6 | 1 | reservoir | MPD | 35 (%) |
