252L

GENERATING LIGAND BINDING SITES IN T4 LYSOZYME USING DEFICIENCY-CREATING SUBSTITUTIONS

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1993-03-25
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 32 2 1
Unit cell lengths	60.800, 60.800, 97.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.100
Rwork	0.163
Structure solution method	DIFFERENCE FOURIER
Starting model (for MR)	CYS-FREE WILD TYPE LYSOZYME WITH RESIDUES 106 - 114 DELETED
RMSD bond length	0.015
RMSD bond angle	0.000 *
Data reduction software	SDMS (DETECTOR SYSTEM (NIELSEN))
Data scaling software	SDMS (DETECTOR SYSTEM (NIELSEN))
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.100	2.100
Rmerge	0.049	0.200
Number of reflections	11176
<I/σ(I)>		2
Completeness [%]	85.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	6.7 *	277	Remington, S.J., (1978). J. Mol. Biol., 118, 81. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	20 (mg/ml)
2	1	1		0.55 (M)
3	1	1	mercaptoethanol	14 (mM)
4	1	1		1 (mM)
5	1	1	sodium phospahte	0.01 (M)
6	1	1		2.2 (M)
7	1	1		1.8 (M)