252D
CRYSTAL STRUCTURE OF THE B-DNA DECAMER D(CGCAATTGCG)2; SEQUENCE-DEPENDENT CROSSED HELIX PACKING
Experimental procedure
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS |
Detector technology | AREA DETECTOR |
Collection date | 1995-08-03 |
Detector | XENTRONICS |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 63.630, 24.700, 38.290 |
Unit cell angles | 90.00, 110.27, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.300 |
R-factor | 0.177 |
Rwork | 0.177 |
RMSD bond length | 0.020 |
RMSD bond angle | 29.700 * |
Data reduction software | XENGEN |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 4.000 * | |
High resolution limit [Å] | 2.200 | 2.200 * |
Rmerge | 0.045 * | 0.131 * |
Number of reflections | 2304 | |
Completeness [%] | 78.0 | 40.2 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 10 * | pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 292.00K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
2 | 1 | 1 | MPD | ||
3 | 1 | 1 | MGCL2 | ||
4 | 1 | 1 | NA CACODYLATE | ||
5 | 1 | 1 | HOECHST 33258 | ||
6 | 1 | 2 | WATER | ||
7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | DNA duplex | 3 (mM) | |
2 | 1 | drop | 200 (mM) | ||
3 | 1 | drop | MPD | 40 (%(v/v)) | |
4 | 1 | drop | sodium cacodylate | 30 (mM) | |
5 | 1 | drop | minor groove binding drug Hoechst 33258 | 9.8 (mM) | |
6 | 1 | reservoir | MPD | 40 (%(v/v)) |