252D
CRYSTAL STRUCTURE OF THE B-DNA DECAMER D(CGCAATTGCG)2; SEQUENCE-DEPENDENT CROSSED HELIX PACKING
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Detector technology | AREA DETECTOR |
| Collection date | 1995-08-03 |
| Detector | XENTRONICS |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 63.630, 24.700, 38.290 |
| Unit cell angles | 90.00, 110.27, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.300 |
| R-factor | 0.177 |
| Rwork | 0.177 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 29.700 * |
| Data reduction software | XENGEN |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 4.000 * | |
| High resolution limit [Å] | 2.200 | 2.200 * |
| Rmerge | 0.045 * | 0.131 * |
| Number of reflections | 2304 | |
| Completeness [%] | 78.0 | 40.2 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 10 * | pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 292.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | NA CACODYLATE | ||
| 5 | 1 | 1 | HOECHST 33258 | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA duplex | 3 (mM) | |
| 2 | 1 | drop | 200 (mM) | ||
| 3 | 1 | drop | MPD | 40 (%(v/v)) | |
| 4 | 1 | drop | sodium cacodylate | 30 (mM) | |
| 5 | 1 | drop | minor groove binding drug Hoechst 33258 | 9.8 (mM) | |
| 6 | 1 | reservoir | MPD | 40 (%(v/v)) |
