250L
THE RESPONSE OF T4 LYSOZYME TO LARGE-TO-SMALL SUBSTITUTIONS WITHIN THE CORE AND ITS RELATION TO THE HYDROPHOBIC EFFECT
Experimental procedure
| Temperature [K] | 300 |
| Detector technology | AREA DETECTOR |
| Collection date | 1994-11-21 |
| Detector | XUONG-HAMLIN MULTIWIRE |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 61.000, 61.000, 96.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| Rwork | 0.160 |
| Structure solution method | DIFFERENCE FOURIER |
| RMSD bond length | 0.014 |
| RMSD bond angle | 15.250 * |
| Data reduction software | UCSD |
| Data scaling software | UCSD |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.042 * |
| Number of reflections | 19250 |
| <I/σ(I)> | 14.3 |
| Completeness [%] | 90.0 |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 6.7 * | Remington, S.J., (1978) J. Mol. Biol., 118, 81. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 20 (mg/ml) | |
| 2 | 1 | 1 | 0.55 (M) | ||
| 3 | 1 | 1 | mercaptoethanol | 14 (mM) | |
| 4 | 1 | 1 | 1 (mM) | ||
| 5 | 1 | 1 | sodium phospahte | 0.01 (M) | |
| 6 | 1 | 1 | 2.2 (M) | ||
| 7 | 1 | 1 | 1.8 (M) |
