24ZP
The alpha amylase AmyY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.441, 71.376, 146.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.530 - 1.500 |
| R-factor | 0.1497 |
| Rwork | 0.149 |
| R-free | 0.17040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.038 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.17.1_3660: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.440 | 1.520 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 83330 | 3678 |
| <I/σ(I)> | 14.3 | |
| Completeness [%] | 99.0 | 90.3 |
| Redundancy | 8.9 | 5.9 |
| CC(1/2) | 0.993 | 0.969 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 0.2 M sodium fluoride, 0.1 M Bis-Tris propane, 20% (w/v) PEG3350 |
