24XN
Structure of WDR5 F263A variant in complex with MBD3C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2026-01-08 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.814, 47.140, 103.097 |
| Unit cell angles | 90.00, 107.48, 90.00 |
Refinement procedure
| Resolution | 49.170 - 1.570 |
| R-factor | 0.1938 |
| Rwork | 0.193 |
| R-free | 0.21170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.865 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.650 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.154 | 1.654 |
| Rmeas | 0.174 | 1.897 |
| Rpim | 0.079 | 0.911 |
| Total number of observations | 51995 | |
| Number of reflections | 83721 | 12111 |
| <I/σ(I)> | 5.5 | 1.4 |
| Completeness [%] | 100.0 | |
| Redundancy | 4.8 | 4.3 |
| CC(1/2) | 0.989 | 0.506 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.15 ammonium sulfate, 0.1 M sodium HEPES, pH7.0, 20% w/v PEG 4000 |
