24QJ
Crystal structure of a tailspike depolymerase (Solidus_gp83) from Acinetobacter phage Solidus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.87313 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 84.224, 84.224, 529.394 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.170 - 2.490 |
| R-factor | 0.21093 |
| Rwork | 0.207 |
| R-free | 0.27673 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.207 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.120 | 2.600 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.215 | 1.540 |
| Rmeas | 0.233 | 1.667 |
| Rpim | 0.088 | 0.626 |
| Total number of observations | 177979 | 18667 |
| Number of reflections | 25822 | 2734 |
| <I/σ(I)> | 7.6 | 1.4 |
| Completeness [%] | 99.1 | |
| Redundancy | 6.9 | 6.8 |
| CC(1/2) | 0.992 | 0.495 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 288 | 0.8M K-Na-tartrate, 0.1M Tris pH 8.5 |
