24QI

Crystal structure of a tailspike depolymerase (Belisarius_gp86) from Acinetobacter phage Belisarius

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS SIRIUS BEAMLINE MANACA
Synchrotron site	LNLS SIRIUS
Beamline	MANACA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-28
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.977180
Spacegroup name	H 3 2
Unit cell lengths	100.975, 100.975, 784.525
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.670 - 2.200
R-factor	0.21119
Rwork	0.210
R-free	0.22610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.924
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.670	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.305	1.936
Rmeas	0.317	2.007
Rpim	0.084	0.523
Total number of observations	1138450	65047
Number of reflections	79321	4496
<I/σ(I)>	8.2	1.7
Completeness [%]	99.9
Redundancy	14.4	14.5
CC(1/2)	0.957	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		288	Bis-tris pH 5.5, 2.4M NaCl