24MO
Cryo-EM structure of AcrB in liposomes incubated with doxorubicin (class1-out)
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.8 |
| Number of Particle | 839760 |
| Software | cryoSPARC,PHENIX,SerialEM,Coot,ISOLDE |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
