24JD
Crystal structure of the Aeropyrum pernix PCNA2 homotrimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-13 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 169.788, 169.788, 169.788 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.090 - 2.170 |
| R-factor | 0.2063 |
| Rwork | 0.205 |
| R-free | 0.23660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.715 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.090 | 2.250 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Rmerge | 0.095 | 0.661 |
| Rmeas | 0.098 | 0.686 |
| Rpim | 0.022 | 0.180 |
| Number of reflections | 167217 | 8530 |
| <I/σ(I)> | 24.82 | 4.23 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 18.8 | 14.3 |
| CC(1/2) | 0.999 | 0.933 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 0.1 M citric acid, 1.8 M ammonium sulfate |
