24IO
Crystal structure of the RelSeq N-terminal domain from Streptococcus equisimilis in complex with pppGpp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | RIGAKU PhotonJet-S |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-12-12 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 173.751, 44.985, 126.433 |
| Unit cell angles | 90.00, 110.08, 90.00 |
Refinement procedure
| Resolution | 58.520 - 3.200 |
| R-factor | 0.2447 |
| Rwork | 0.242 |
| R-free | 0.29870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.795 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 118.700 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 14251 | 713 |
| <I/σ(I)> | 4.2 | |
| Completeness [%] | 90.9 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.838 | 0.522 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1M Tris-HCl, pH 8.5, 24% PEG8000, 1M NaCl |
