24IL
alpha-1,2-glucosidase from Arthrobacter humicola A8F5, selaginose complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-11-10 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.803, 108.080, 105.435 |
| Unit cell angles | 90.00, 93.28, 90.00 |
Refinement procedure
| Resolution | 48.160 - 1.800 |
| R-factor | 0.20138 |
| Rwork | 0.199 |
| R-free | 0.23985 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AlphaFold |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.305 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.520 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.045 | 0.515 |
| Rmeas | 0.054 | 0.613 |
| Number of reflections | 114361 | 5627 |
| <I/σ(I)> | 14.3 | 2.1 |
| Completeness [%] | 99.1 | 99.7 |
| Redundancy | 3.3 | 3.4 |
| CC(1/2) | 0.999 | 0.848 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 293 | 0.1 M sodium acetate pH5.1 , 34%(v/v)1,2-propanediol |
