24GZ

Artificial Copper-binding trimeric protein 1 (Cu(I)TP6)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-12-18
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979
Spacegroup name	P 3
Unit cell lengths	115.256, 115.256, 38.969
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.810 - 2.340
R-factor	0.2114
Rwork	0.209
R-free	0.26730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.424
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.810	2.430
High resolution limit [Å]	2.340	2.340
Number of reflections	23966	2369
<I/σ(I)>	11.53
Completeness [%]	98.0
Redundancy	10.5
CC(1/2)	0.988	0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1 M Tris-HCl (pH 8.5) buffer containing 0.2 M trimethylamine N-oxide and 15% (w/v) PEG 2000 MME