24GF
Crystal structure of HpsK from Ruegeria pomeroyi in complex with R-DHPS (2,3-dihydroxypropanesulfonate)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-08-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.954 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 84.791, 69.464, 71.473 |
| Unit cell angles | 90.00, 120.42, 90.00 |
Refinement procedure
| Resolution | 42.218 - 1.958 |
| Rwork | 0.144 |
| R-free | 0.17790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.427 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0431) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.070 | 2.010 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Number of reflections | 25729 | 1747 |
| <I/σ(I)> | 12.1 | |
| Completeness [%] | 99.7 | |
| Redundancy | 3.5 | |
| CC(1/2) | 1.000 | 0.930 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG3350, BIS-TRIS, pH 6.5 |
