24GE
Crystal structure of HpsK from Ruegeria pomeroyi in complex with S-DHPS (2,3-dihydroxypropanesulfonate)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-10-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.954 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 84.793, 68.756, 71.377 |
| Unit cell angles | 90.00, 120.32, 90.00 |
Refinement procedure
| Resolution | 42.208 - 1.650 |
| Rwork | 0.141 |
| R-free | 0.16390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.498 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0431) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.208 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 42559 | 2071 |
| <I/σ(I)> | 16.1 | |
| Completeness [%] | 99.9 | |
| Redundancy | 5.2 | |
| CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG3350, Bis-Tris, pH 6.5 |
