24BX

Crystal structure of nuclease MYG1(H50A) bound to Mn2+ and AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	193
Detector technology	PIXEL
Collection date	2025-11-29
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.9786
Spacegroup name	C 2 2 21
Unit cell lengths	70.234, 97.155, 116.842
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.920 - 2.010
R-factor	0.1818
Rwork	0.180
R-free	0.20940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.866
Data reduction software	HKL-2000 (1.20.1_4487)
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.920	2.080
High resolution limit [Å]	2.010	2.010
Number of reflections	27166	3890
<I/σ(I)>	13.3
Completeness [%]	100.0
Redundancy	12.6
CC(1/2)	0.990	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M sodium citrate tribasic dihydrate, pH 5.5, 16% w/v PEG 8000