24BW

Crystal structure of nuclease MYG1(D93A) bound to Mn2+ and UU

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL18U
Synchrotron site	NFPSS
Beamline	BL18U
Temperature [K]	193
Detector technology	PIXEL
Collection date	2024-10-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9786
Spacegroup name	C 2 2 21
Unit cell lengths	69.678, 98.036, 116.695
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.380 - 1.820
R-factor	0.1983
Rwork	0.197
R-free	0.22340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.881
Data reduction software	HKL-2000 (1.20.1_4487)
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.380	1.880
High resolution limit [Å]	1.820	1.820
Number of reflections	36379	5233
<I/σ(I)>	19.9
Completeness [%]	100.0
Redundancy	13
CC(1/2)	0.990	0.910

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M sodium citrate, pH 5.5, 20% w/v PEG 3000