24BV

Crystal structure of nuclease MYG1(D176A) bound to Mn2+ and AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	193
Detector technology	PIXEL
Collection date	2025-11-29
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.9786
Spacegroup name	C 2 2 21
Unit cell lengths	69.469, 97.443, 116.793
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.360 - 2.450
R-factor	0.2028
Rwork	0.197
R-free	0.25110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.930
Data reduction software	HKL-2000 (1.20.1_4487)
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.360	2.530
High resolution limit [Å]	2.450	2.450
Number of reflections	14552	1653
<I/σ(I)>	16.4
Completeness [%]	97.3
Redundancy	12.9
CC(1/2)	0.990	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M sodium citrate, pH 5.0, 20% w/v PEG 8000