24BU

Crystal structure of nuclease MYG1 bound to Mn2+ and dGMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	193
Detector technology	PIXEL
Collection date	2024-12-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9791
Spacegroup name	C 2 2 21
Unit cell lengths	70.529, 97.497, 116.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.150 - 1.930
R-factor	0.1712
Rwork	0.169
R-free	0.19680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.776
Data reduction software	HKL-2000 (1.20.1_4487)
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.150	1.990
High resolution limit [Å]	1.930	1.930
Number of reflections	30657	2012
<I/σ(I)>	14.2
Completeness [%]	99.9
Redundancy	10.5
CC(1/2)	0.990	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.2 M ammonium tartrate dibasic, pH 6.6, 20% w/v PEG 3350