24BT
Crystal structure of Peptidyl-tRNA Hydrolase 2 from Candidatus Lokiarchaeum sp. GC14_75
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2024-10-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 72.930, 42.174, 156.520 |
| Unit cell angles | 90.00, 90.12, 90.00 |
Refinement procedure
| Resolution | 39.130 - 2.120 |
| R-factor | 0.20028 |
| Rwork | 0.199 |
| R-free | 0.21992 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.354 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.130 | 2.250 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Rmerge | 0.053 | 0.169 |
| Number of reflections | 26728 | 4253 |
| <I/σ(I)> | 15 | |
| Completeness [%] | 97.3 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 50 mM HEPES-NaOH pH 7.4, 19% (w/v) PEG3350, and 0.2 M Ammonium citrate dibasic |
