24BS

Crystal structure of nuclease MYG1 bound to Mn2+ and dAMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	193
Detector technology	PIXEL
Collection date	2024-12-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9791
Spacegroup name	C 2 2 21
Unit cell lengths	69.698, 97.870, 116.897
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.980 - 2.000
R-factor	0.1715
Rwork	0.169
R-free	0.19950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.804
Data reduction software	HKL-2000 (1.20.1_4487)
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.980	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	26717	1577
<I/σ(I)>	13.7
Completeness [%]	97.7
Redundancy	11.4
CC(1/2)	0.990	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M magnesium acetate, 0.1 M sodium cacodylate, pH 6.5, 15% w/v PEG 6000