24BR

Crystal structure of nuclease MYG1(D57A) bound to Mn2+ and UUUU

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	193
Detector technology	PIXEL
Collection date	2025-01-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9791
Spacegroup name	C 2 2 21
Unit cell lengths	70.510, 97.560, 116.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.740 - 2.100
R-factor	0.1872
Rwork	0.185
R-free	0.22420
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.803
Data reduction software	HKL-2000 (1.20.1_4487)
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.740	2.170
High resolution limit [Å]	2.100	2.100
Number of reflections	23523	1580
<I/σ(I)>	13.1
Completeness [%]	98.7
Redundancy	10.7
CC(1/2)	0.990	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.2 M ammonium sulfate, 0.1 M Tris, pH 5.5, 25% w/v PEG 3350