24BP

Crystal structure of nuclease MYG1 bound to Mn2+ and dAC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL18U
Synchrotron site	NFPSS
Beamline	BL18U
Temperature [K]	193
Detector technology	PIXEL
Collection date	2024-11-29
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	C 2 2 21
Unit cell lengths	69.938, 97.562, 116.503
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.720 - 2.200
R-factor	0.1849
Rwork	0.181
R-free	0.22420
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.836
Data reduction software	HKL-2000 (1.20.1_4487)
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.720	2.270
High resolution limit [Å]	2.200	2.200
Number of reflections	20602	1774
<I/σ(I)>	14.3
Completeness [%]	99.9
Redundancy	10.5
CC(1/2)	0.990	0.860

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M sodium citrate, pH 5.5, 20% w/v PEG 3000