24BM

Crystal structure of nuclease MYG1(H107A) bound to Mn2+ and AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	193
Detector technology	PIXEL
Collection date	2025-12-26
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.9786
Spacegroup name	C 2 2 21
Unit cell lengths	69.669, 96.947, 115.975
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.580 - 2.310
R-factor	0.1964
Rwork	0.192
R-free	0.24020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.823
Data reduction software	HKL-2000 (1.20.1_4487)
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.580	2.390
High resolution limit [Å]	2.310	2.310
Number of reflections	17529	2503
<I/σ(I)>	8.8
Completeness [%]	99.9
Redundancy	9.2
CC(1/2)	0.990	0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.15 M ammonium sulfate, 0.1 M MES, pH 6.0, 15% w/v PEG 4000