24BD

Crystal structure of nuclease MYG1(D57A) bound to Mn2+ and AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	193
Detector technology	PIXEL
Collection date	2026-01-25
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.9786
Spacegroup name	C 2 2 21
Unit cell lengths	70.511, 97.620, 116.931
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.470 - 1.780
R-factor	0.1822
Rwork	0.181
R-free	0.20490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.808
Data reduction software	HKL-2000 (1.20.1_4487)
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.470	1.840
High resolution limit [Å]	1.780	1.780
Number of reflections	38366	5615
<I/σ(I)>	10.6
Completeness [%]	98.2
Redundancy	13.2
CC(1/2)	0.990	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.2 M lithium sulfate, 0.1 M MES, pH 6.0, 20% w/v PEG 4000