24AZ
alpha-1,2-glucosidase from Arthrobacter humicola A8F5, kojitriose complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-12-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.385, 107.985, 105.522 |
| Unit cell angles | 90.00, 93.34, 90.00 |
Refinement procedure
| Resolution | 48.490 - 1.830 |
| R-factor | 0.21272 |
| Rwork | 0.210 |
| R-free | 0.25720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AlphaFold |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.337 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.490 | 1.860 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.267 | 3.160 |
| Rmeas | 0.277 | 3.277 |
| Number of reflections | 111405 | 5305 |
| <I/σ(I)> | 7.8 | 1 |
| Completeness [%] | 99.8 | 96.9 |
| Redundancy | 14.1 | 14.3 |
| CC(1/2) | 0.995 | 0.567 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 293 | 0.1 M sodium acetate pH5.1 , 34%(v/v)1,2-propanediol |
