24AY

Crystal structure of nuclease MYG1 bound to Mn2+ and dTMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	193
Detector technology	PIXEL
Collection date	2024-11-18
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.9786
Spacegroup name	C 2 2 21
Unit cell lengths	70.146, 98.108, 116.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.380 - 2.120
R-factor	0.1872
Rwork	0.184
R-free	0.22290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.820
Data reduction software	HKL-2000 (1.20.1_4487)
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.380	2.190
High resolution limit [Å]	2.120	2.120
Number of reflections	22509	3370
<I/σ(I)>	13.4
Completeness [%]	96.7
Redundancy	8.3
CC(1/2)	0.990	0.860

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M Bis-Tris propane, pH 9.0, 10% w/v Jeffamine ED-2001, pH 7.0