24AO

Crystal structure of nuclease MYG1 bound to Mn2+ and GMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	193
Detector technology	PIXEL
Collection date	2025-11-29
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.9786
Spacegroup name	C 2 2 21
Unit cell lengths	70.530, 97.069, 116.762
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.060 - 1.810
R-factor	0.1752
Rwork	0.173
R-free	0.20270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.760
Data reduction software	HKL-2000 (1.20.1_4487)
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.060	1.870
High resolution limit [Å]	1.810	1.810
Number of reflections	33925	5380
<I/σ(I)>	16.2
Completeness [%]	91.2
Redundancy	12.8
CC(1/2)	0.990	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.2 M ammonium sulfate, 0.1 M Tris pH 6.5, 25% w/v PEG 3350