24AJ

Crystal structure of nuclease MYG1 bound to Na+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	193
Detector technology	PIXEL
Collection date	2024-12-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9791
Spacegroup name	C 2 2 21
Unit cell lengths	70.150, 97.730, 116.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.870 - 1.830
R-factor	0.1868
Rwork	0.185
R-free	0.21830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.793
Data reduction software	HKL-2000 (1.20.1_4487)
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.870	1.890
High resolution limit [Å]	1.830	1.830
Number of reflections	35587	2562
<I/σ(I)>	18.4
Completeness [%]	99.5
Redundancy	10.8
CC(1/2)	0.990	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M sodium citrate tribasic dihydrate, pH 5.5, 18% w/v PEG 3350