24PU

The crystal structure of the chicken FANCM-MHF1-MHF2(Q74C) disulfide-crosslinked complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	59.527, 90.452, 92.955
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.480 - 2.400
R-factor	0.2129
Rwork	0.206
R-free	0.27380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.911
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.480	2.486
High resolution limit [Å]	2.400	2.400
Rmerge	0.155	1.516
Rmeas	0.169	1.676
Rpim	0.065	0.700
Number of reflections	37521	1882
<I/σ(I)>	8.87	0.88
Completeness [%]	99.5	95.34
Redundancy	6.5	5.4
CC(1/2)	0.996	0.428

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.7	293	0.1M Tris Bicine pH 8.7, 50mM Carboxylic acid mix, 15% MPD, 20% PEG 3350, 100mM NaCl