249D
STRUCTURAL COMPARISON BETWEEN THE D(CTAG) SEQUENCE IN OLIGONUCLEOTIDES AND TRP AND MET REPRESSOR-OPERATOR COMPLEXES
Experimental procedure
| Detector technology | DIFFRACTOMETER |
| Collection date | 1989-04-03 |
| Detector | ENRAF-NONIUS CAD4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.050, 68.910, 26.150 |
| Unit cell angles | 90.00, 91.16, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.250 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 4.100 |
| Data reduction software | ENRAF-NONIUS |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.250 |
| Rmerge | 0.090 |
| Number of reflections | 5276 |
| Completeness [%] | 88.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | pH 7.00, VAPOR DIFFUSION |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | CACL2 | ||
| 4 | 1 | 2 | WATER | ||
| 5 | 1 | 2 | MPD |
