247D
CRYSTAL STRUCTURES OF AN A-FORM DUPLEX WITH SINGLE-ADENOSINE BULGES AND A CONFORMATIONAL BASIS FOR SITE SPECIFIC RNA SELF-CLEAVAGE
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1994-06-13 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 27.245, 27.245, 177.399 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.800 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.950 |
| Data reduction software | R-AXIS |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.570 | |
| High resolution limit [Å] | 2.800 | 2.800 * |
| Rmerge | 0.075 | |
| Number of reflections | 1035 | |
| Completeness [%] | 82.0 | 50 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.9 * | pH 7.40, VAPOR DIFFUSION |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | NA CACODYLATE | ||
| 3 | 1 | 1 | SPERMINE | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | oligonucleotide | 0.8 (mM) | ||
| 2 | 1 | sodium cacodylate | 15 (mM) | ||
| 3 | 1 | spermine tetrahydrochloride | 50 (mM) | ||
| 4 | 1 | 2 | MPD | 40 (%) |
