246D
STRUCTURE OF THE PURINE-PYRIMIDINE ALTERNATING RNA DOUBLE HELIX, R(GUAUAUA)D(C) , WITH A 3'-TERMINAL DEOXY RESIDUE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 295 |
| Detector technology | AREA DETECTOR |
| Collection date | 1994-07-17 |
| Detector | SIEMENS |
| Spacegroup name | H 3 |
| Unit cell lengths | 43.100, 43.100, 59.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.200 |
| R-factor | 0.156 |
| Rwork | 0.156 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.550 |
| Data reduction software | XENGEN (V. 2.0) |
| Refinement software | X-PLOR (3.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.000 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.017 |
| Number of reflections | 1775 |
| Completeness [%] | 85.5 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 * | pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 291.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | [CO(NH3)6]3+ | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | oligonucleotide | 2 (mM) | |
| 2 | 1 | drop | cobaltic hexamine trichloride | 6 (mM) | 0.5 ml |
| 3 | 1 | drop | sodium cacodylate | 100 (mM) | 0.001 ml |
| 4 | 1 | drop | water | 0.005 ml | |
| 5 | 1 | drop | MPD | 0.001 ml | |
| 6 | 1 | reservoir | 60 (%) |
