243D
STRUCTURE OF THE DNA OCTANUCLEOTIDE D(ACGTACGT)2
Experimental procedure
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-10-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 42.845, 42.845, 24.804 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 5.000 - 1.900 |
| R-factor | 0.177 |
| Rwork | 0.177 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 3.470 |
| Data reduction software | XDS |
| Refinement software | X-PLOR (3.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 9.000 |
| High resolution limit [Å] | 1.500 |
| Rmerge | 0.088 |
| Number of reflections | 3962 |
| Completeness [%] | 88.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 291.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | CACODYLATE | ||
| 4 | 1 | 1 | MG ACETATE | ||
| 5 | 1 | 1 | SPERMINE_HCL | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 0.5 (mM) | |
| 2 | 1 | drop | cacodylate | 1.5 (mM) | |
| 3 | 1 | drop | magnesium acetate | 1.25 (mM) | |
| 4 | 1 | drop | sperine-4HCl | 2.5 (mM) | |
| 5 | 1 | drop | MPD | 6 (%) | |
| 6 | 1 | reservoir | MPD | 30 (%) |
