241D
EXTENSION OF THE FOUR-STRANDED INTERCALATED CYTOSINE MOTIF BY ADENINE.ADENINE BASE PAIRING IN THE CRYSTAL STRUCTURE OF D(CCCAAT)
Experimental procedure
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 62 |
| Unit cell lengths | 32.180, 32.180, 52.490 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.850 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| RMSD bond length | 0.028 |
| RMSD bond angle | 3.700 |
| Refinement software | X-PLOR |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 4 * | pH 6.00, VAPOR DIFFUSION, HANGING DROP, temperature 277.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | SPERMINE | ||
| 4 | 1 | 1 | NA CACODYLATE | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | MPD | 5 (%) | |
| 2 | 1 | drop | spermine | 0.1 (mM) | |
| 3 | 1 | drop | Na-cacodylate | 80 (mM) | |
| 4 | 1 | drop | DNA | 2 (mM) | |
| 5 | 1 | reservoir | MPD | 35 (%) |
