23TK

Structure of the 328-692 fragment of FlhA (A489E) form 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	50.240, 93.250, 187.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.520 - 2.900
R-factor	0.2526
Rwork	0.252
R-free	0.26200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ai0
RMSD bond length	0.003
RMSD bond angle	0.689
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.200	3.080
High resolution limit [Å]	2.900	2.900
Rmerge	0.107	0.479
Number of reflections	20281	3194
<I/σ(I)>	8.4	2.3
Completeness [%]	99.9
Redundancy	5.1
CC(1/2)	0.994	0.936

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	293	10% PEG3000, 0.1M KH2PO4 / Na2HPO4, 10% glycerol. Protein conc. : 10 mg/mL