23SG
The composite Cryo-EM structure of bacteriophage RAN69 pre-ejectosome-portal complex
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | TFS FALCON 4i (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3 |
| Number of Particle | 53029 |
| Software | RELION,CTFFIND |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
