23EQ

Artificial Copper-binding trimeric protein 1 (Cu(I)TP1)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-10-12
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	45.860, 78.480, 85.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.100 - 1.810
R-factor	0.2315
Rwork	0.230
R-free	0.25910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.809
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.100	1.875
High resolution limit [Å]	1.810	1.810
Number of reflections	28905	2834
<I/σ(I)>	18.31
Completeness [%]	99.8
Redundancy	12.5
CC(1/2)	0.999	0.876

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1 M Bis-Tris (pH 7.0) buffer containing 0.4 M NaCl and 27.5% (w/v) PEG