23AG

Crystal structure of BAZ2A in complex with an S-HDAg_K72ac peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000000
Spacegroup name	P 31 2 1
Unit cell lengths	95.426, 95.426, 33.155
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	82.640 - 1.610
R-factor	0.1858
Rwork	0.185
R-free	0.20390
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.811
Data reduction software	DIALS
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.640	1.640
High resolution limit [Å]	1.610	1.610
Number of reflections	22413	984
<I/σ(I)>	10.4
Completeness [%]	98.7
Redundancy	14.5
CC(1/2)	0.994	0.642

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		289	20% PEG 3350, 0.2 M ammonium formate