23AA

Type II-A Anti-CRISPR protein AcrIIA17 from Streptococcus gallolyticus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	125
Detector technology	PIXEL
Collection date	2022-09-25
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	45.080, 57.670, 81.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.840 - 2.030
R-factor	0.2256
Rwork	0.222
R-free	0.25430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.160
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.840	2.100
High resolution limit [Å]	2.030	2.030
Number of reflections	14137	1369
<I/σ(I)>	18.06
Completeness [%]	99.4
Redundancy	13.2
CC(1/2)	1.000	0.742

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	30% (v/v) 2-Propanol, 100 mM Tris base/ Hydrochloric acid pH 8.5, 30% (w/v) PEG 3350