23VF

Crystal structure of CEACAM1 in complex with MG1124 antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	70.985, 140.722, 206.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.448 - 1.793
R-factor	0.2287
Rwork	0.228
R-free	0.25760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4whd 4evn
RMSD bond length	0.008
RMSD bond angle	0.979
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.850
High resolution limit [Å]	1.790	1.790
Rmerge	0.099	0.270
Number of reflections	142860	5369
<I/σ(I)>	14.6	1.9
Completeness [%]	73.8	23.8
Redundancy	5.6	1.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	36.6mg/mL of protein complex 0.1M Lithium sulfate monohydrate 0.1M ADA (pH6.5) 14%(w/v) PEG 4000 2%(v/v) Iso-propanol 17.5% Ethylene glycol for cryoprotection