237D
CRYSTAL STRUCTURE OF A DNA DECAMER SHOWING A NOVEL PSEUDO FOUR-WAY HELIX-HELIX JUNCTION
Experimental procedure
| Source type | ROTATING ANODE |
| Temperature [K] | 294 |
| Detector technology | AREA DETECTOR |
| Detector | XENTRONICS |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 27.010, 39.260, 54.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.300 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.102 * |
| Number of reflections | 1521 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 287 | pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 287.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | NA CACODYLATE | ||
| 5 | 1 | 1 | BERENIL | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium cacodylate | 30 (mM) | |
| 2 | 1 | drop | 2.0 (M) | 0.001ml | |
| 3 | 1 | drop | MPD | 49 (%) | 0.003ml |
| 4 | 1 | drop | decamer | 3 (mM) | 0.004ml |
| 5 | 1 | drop | minor-groove-binding drug berenil | 15 (mM) | 0.002ml |
| 6 | 1 | reservoir | MPD | 50 (%) |
