230L
T4 LYSOZYME MUTANT M6L
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-01 |
| Detector | XUONG-HAMLIN MULTIWIRE |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.870, 60.870, 97.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| Rwork | 0.184 |
| Structure solution method | DIFFERENCE FOURIER |
| RMSD bond length | 0.016 |
| RMSD bond angle | 15.710 * |
| Data reduction software | SDMS (DETECTOR SYSTEM (NIELSEN)) |
| Data scaling software | SDMS (DETECTOR SYSTEM (NIELSEN)) |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.760 |
| Rmerge | 0.077 | 0.214 |
| Number of reflections | 16280 | |
| <I/σ(I)> | 13.1 | 1.9 |
| Completeness [%] | 77.0 | 45.6 |
| Redundancy | 2 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 * | 4 * | M6L WAS AT 15MG/ML IN A BUFFER CONTAINING 0.1M NA2PO4 PH 6.6, 0.55 M NACL. IT WAS DILUTED BY 1/2 WITH A SOLUTION 1.8M IN NA/KPO4 PH 6.9. THIS WAS ALSO THE WELL SOLUTION. HANGING DROP METHODS WERE USED., pH 7.0, vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium phosphate | 0.1 (M) | |
| 2 | 1 | drop | 0.55 (M) | ||
| 3 | 1 | drop | 0.02 (%) | ||
| 4 | 1 | reservoir | oxidized beta-mercaptoethanol | 50 (mM) | |
| 5 | 1 | reservoir | reduced beta-mercaptoethanol | 50 (mM) | |
| 6 | 1 | reservoir | sodium/potassium phosphate | 1.8-2.0 (M) |
