230L

T4 LYSOZYME MUTANT M6L

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1996-01
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 32 2 1
Unit cell lengths	60.870, 60.870, 97.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.900
Rwork	0.184
Structure solution method	DIFFERENCE FOURIER
RMSD bond length	0.016
RMSD bond angle	15.710 *
Data reduction software	SDMS (DETECTOR SYSTEM (NIELSEN))
Data scaling software	SDMS (DETECTOR SYSTEM (NIELSEN))
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.900
High resolution limit [Å]	1.800	1.760
Rmerge	0.077	0.214
Number of reflections	16280
<I/σ(I)>	13.1	1.9
Completeness [%]	77.0	45.6
Redundancy	2	1.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.1 *	4 *	M6L WAS AT 15MG/ML IN A BUFFER CONTAINING 0.1M NA2PO4 PH 6.6, 0.55 M NACL. IT WAS DILUTED BY 1/2 WITH A SOLUTION 1.8M IN NA/KPO4 PH 6.9. THIS WAS ALSO THE WELL SOLUTION. HANGING DROP METHODS WERE USED., pH 7.0, vapor diffusion - hanging drop

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	sodium phosphate	0.1 (M)
2	1	drop		0.55 (M)
3	1	drop		0.02 (%)
4	1	reservoir	oxidized beta-mercaptoethanol	50 (mM)
5	1	reservoir	reduced beta-mercaptoethanol	50 (mM)
6	1	reservoir	sodium/potassium phosphate	1.8-2.0 (M)