22VH
The assembled CPC trimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-12-09 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.970, 109.960, 182.370 |
| Unit cell angles | 90.00, 103.47, 90.00 |
Refinement procedure
| Resolution | 54.270 - 2.500 |
| R-factor | 0.1962 |
| Rwork | 0.194 |
| R-free | 0.24610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.577 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.270 | 2.550 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.280 | |
| Number of reflections | 78269 | 6070 |
| <I/σ(I)> | 9 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.6 | |
| CC(1/2) | 0.999 | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1 M tribasic sodium citrate dihydrate (pH 5.5) and 18% w/v polyethylene glycol 3350 |
