22PE
Ethylene Forming Enzyme in complex with 2-oxoglutarate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9779 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 79.366, 97.751, 98.246 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.880 - 1.660 |
| R-factor | 0.1654 |
| Rwork | 0.164 |
| R-free | 0.19290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.254 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.880 | 1.700 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.124 | 0.822 |
| Rpim | 0.041 | 0.362 |
| Number of reflections | 45253 | 2109 |
| <I/σ(I)> | 16.97 | 1.63 |
| Completeness [%] | 99.6 | 95.4 |
| Redundancy | 9.7 | 5.3 |
| CC(1/2) | 0.626 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289.15 | 0.1 M sodium cacodylate pH 6.5, 25% (w/v) polyethylene glycol (PEG) 4000 |
