22MX

Crystal structure of a variant c-di-GMP-II Riboswitch c-di-GMP-II-Bh_A9G/U55C bound with 2',3'-cGAMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.10202
Spacegroup name	P 63
Unit cell lengths	93.228, 93.228, 102.372
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.700 - 2.900
R-factor	0.2034
Rwork	0.197
R-free	0.25850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.153
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.800	3.100
High resolution limit [Å]	2.900	2.900
Number of reflections	11215	11215
<I/σ(I)>	12.8
Completeness [%]	100.0
Redundancy	18.9
CC(1/2)	1.000	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2M NH4Ac, 0.1M Tris 8.5, 45% MPD