22LY
De novo designed S-locus Protein 11 (SP11)-like protein (P212121 form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 80.234, 82.828, 118.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.160 - 2.100 |
| R-factor | 0.20197 |
| Rwork | 0.200 |
| R-free | 0.23925 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.682 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0431) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.160 | 2.220 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmeas | 0.190 | 1.497 |
| Number of reflections | 46962 | 7418 |
| <I/σ(I)> | 24.5 | 3.3 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 27 | |
| CC(1/2) | 0.999 | 0.918 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.5M di-ammonium tartrate, 17.5% (w/v) PEG 3350 |
