22LX
De novo designed S-locus Protein 11 (SP11)-like protein (P6522 form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-20 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 64.300, 64.300, 126.221 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 51.000 - 1.500 |
| R-factor | 0.18119 |
| Rwork | 0.180 |
| R-free | 0.21339 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.181 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.061 | 0.965 |
| Rmeas | 0.063 | 1.050 |
| Rpim | 0.015 | 0.399 |
| Number of reflections | 25451 | 1140 |
| <I/σ(I)> | 25.1 | 1.6 |
| Completeness [%] | 99.6 | |
| Redundancy | 16.8 | |
| CC(1/2) | 0.999 | 0.609 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.4 | 293.15 | 0.16M ammonium sulfate, 0.08M Na-acetate (pH 4.4), 20% (v/v) glycerol |
