22IY
Crystal structure of the unliganded microalgal AstaP-pink1 carotenoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS SIRIUS BEAMLINE MANACA |
| Synchrotron site | LNLS SIRIUS |
| Beamline | MANACA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-02-28 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9772 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.483, 56.525, 58.692 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.710 - 1.650 |
| R-factor | 0.17827 |
| Rwork | 0.176 |
| R-free | 0.22806 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.216 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0431) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.710 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.193 | 3.096 |
| Rmeas | 0.203 | 3.256 |
| Rpim | 0.062 | 0.996 |
| Total number of observations | 176663 | 8527 |
| Number of reflections | 16831 | 821 |
| <I/σ(I)> | 8.8 | 0.9 |
| Completeness [%] | 100.0 | |
| Redundancy | 10.5 | 10.4 |
| CC(1/2) | 0.998 | 0.416 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1M Tris pH 8.5, 25% w/v Polyethylene glycol 3350 |
